HUMAN GROWTH HORMONE
99%
somatropin
The name “Somatropin 99%” speaks for itself and means a high degree of purification of the product. The purity of the product is controlled according to all required parameters: protein, lipids, degree of pyrogenicity, etc. Thanks to the use of modern technologies and patented production methods, the product has high biological activity, which is regularly tested on eukaryotic cell cultures.
Areas of use:
- increased protein synthesis;
- stimulation of anabolic processes;
- strengthening the muscle structure and ligamentous apparatus;
- reducing the percentage of visceral and subcutaneous fat;
- regeneration after injuries;
- strengthening the immune system;
- rejuvenation of the body (can be seen by a decrease in the rate of shortening of heat meters during histological analysis);
- treatment of diseases of the nervous system.
* Consult your healthcare practitioner before use
How is recombinant growth hormone produced
Synthesized growth hormone was first obtained in 1985 based on recombinant DNA with an amino acid sequence identical to human growth hormone. Since that time, studies have begun to study the effects of growth hormone replacement therapy in adults. Recombinant growth hormone was finally approved for growth hormone deficiency in adults in the 1990s.
Somatotropin in its structure is a polypeptide consisting of 191 amino acid residues and having two disulfide bridges.
The technological cycle for the production of recombinant somatotropin consists of 5 stages:
- construction of recombinant DNA, obtaining a transformed microorganism cell;
- fermentation;
- isolation and purification: primary protein purification, chromatographic purification;
- production of dosage form;
- analysis of the quality of the substance and dosage form of somatotropin.
At the first stage, a double-stranded DNA copy of the mRNA is cloned and, by digestion with restriction endonucleases, a sequence is obtained that encodes the entire amino acid sequence of the hormone, with the exception of the first 23 amino acids. Then a polypeptide corresponding to amino acids 1 to 23 is synthesized using a chemical method. Next, the two fragments are combined.
The production process is carried out using aseptic technology without thermal sterilization, with the introduction of certain auxiliary substances that ensure stability, solubility of the substance, and sterility.
Fermentation is carried out in bioreactors by deep cultivation under aerobic conditions on nutrient media traditional for microorganisms. Cell destruction is carried out using a chemical method: sodium bicarbonate solution with pH = 11 (98%).
About growth hormone
The problem of growth hormone deficiency has long been considered as a disease of childhood. However, in recent years, the physiological role of growth hormone in adults has been increasingly discussed. Growth hormone deficiency in adults can manifest itself clinically and lead to serious metabolic disorders that require timely diagnosis and treatment.
Throughout life, growth hormone stimulates chondrogenesis and mineralization of bone tissue, tissue and organ growth, increases muscle anabolism, glycogenolysis and gluconeogenesis, exhibits counterinsular activity, enhances the mobilization of free fatty acids, the absorption of calcium and phosphates in the gastrointestinal tract, increases the reabsorption of phosphates in the kidneys, reduces release of potassium and sodium.
GH has a transient (during the first 30-40 minutes) insulin-like effect on fat metabolism, which is manifested by increased lipogenesis processes. However, in the future, on the contrary, the processes of lipolysis and mobilization of fat from the depot intensify, which leads to an increase in free fatty acids in the blood plasma. The lipolytic effect is due to a decrease in the size of mature adipocytes, which leads to an overall reduction in the volume of adipose tissue.
Lack of GH in the body leads to a decrease in the quality of life and the manifestation of symptoms, the cause of which is often considered to be other diseases. Currently, recombinant growth hormone replacement therapy is increasingly used, which has a wide range of beneficial effects. The use of somatotropic hormone slows down the aging of the body, strengthens the osteochondral structure, and improves the functioning of the cardiovascular system. Somatotropic hormone accelerates the healing of injuries, and also helps to increase muscle mass and reduce the percentage of visceral and subcutaneous fat. This effect is most pronounced in people involved in sports.
* Before starting replacement therapy, consult your doctor and carefully read the instructions for the preparation.